| 质粒名称: | pLenti X2 Hygro DEST (w17-1) |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 17295 |
| 存储实验室: | Eric Campeau, Paul Kaufman |
|
载体骨架基本信息 |
|
| 载体名称: | p156RRL-sinPPT-CMV-GFP-PRE/Nhe I |
| 载体抗性: | 氯霉素和氨苄霉素 |
| 空载体大小: | 9772 bp |
| 载体类型: | Lentiviral, RNAi |
| 筛选标记: | 潮霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | None |
| 克隆方法: | 限制性内切酶 |
| 5' 测序引物 | n/a |
|
文献引用方法 |
|
| 材料和方法部分: | pLenti X2 Hygro DEST (w17-1) was a gift from Vladislav Verkhusha (爱迪基因 plasmid # 17295) |
| 参考文献部分: | A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD.. PLoS One. 2009 Aug 6;4(8):e6529. 10.1371/journal.pone.0006529 PubMed 19657394 |
买家导航
pLenti X2 Hygro DEST (w17-1)载体质粒基本信息
pLenti X2 Hygro DEST (w17-1)质粒图谱载体图谱和pLenti X2 Hygro DEST (w17-1)载体序列质粒序列多克隆位点信息
质粒图谱.png)
pLenti X2 Hygro DEST (w17-1)质粒载体简介
pLenti X2 Hygro DEST (w17-1)质粒序列载体序列
LOCUS pLenti X2 Hygro DEST (w17-1) 9772 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION 3rd gen lentiviral Gateway destination vector, RNAi, 3'LTR
insertion, Hygro.
ACCESSION .
VERSION .
KEYWORDS pLenti X2 Hygro DEST (w17-1)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9772)
AUTHORS Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO,
Campisi J, Yaswen P, Cooper PK, Kaufman PD.
TITLE A versatile viral system for expression and depletion of proteins in
mammalian cells.
JOURNAL PLoS One. 2009 Aug 6;4(8):e6529.
PUBMED 19657394
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pLenti X2 Hygro DEST (w17-1)|
FEATURES Location/Qualifiers
source 1..9772
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 27..131
/gene="bla"
/label=AmpR promoter
CDS 132..992
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(350..369)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 1163..1751
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 1652..1671
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 1905..1922
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 2039..2060
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2075..2105
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2113..2129
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2118..2140
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 2137..2153
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 2137..2153
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 2172..2192
/label=T3
/note="T3 promoter, forward primer"
promoter 2174..2192
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
LTR 2448..2628
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 2672..2797
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 3290..3523
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 4012..4129
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
misc_feature 4183..4771
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4236..4256)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(4654..4665)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
promoter 4827..5326
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(4862..4885)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 5259..5278
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
protein_bind 6787..6911
/gene="mutant version of attR"
/label=attR2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
CDS complement(6952..7257)
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind complement(7054..7073)
/label=ccdB-fwd
/note="ccdB gene, forward primer"
CDS complement(7599..8258)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind 8173..8192
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
promoter complement(8312..8342)
/label=lac UV5 promoter
/note="E. coli lac promoter with an ""up"" mutation"
protein_bind complement(8367..8491)
/gene="mutant version of attR"
/label=attR1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
LTR 8566..8746
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
polyA_signal 8818..8939
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(8855..8874)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 8909..8928
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 8958..9093
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 9020..9039
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(9125..9144)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(9126..9144)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(9154..9171)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(9154..9170)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(9163..9185)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 9315..9770
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(9402..9421)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 9612..9633
/label=F1ori-F
/note="F1 origin, forward primer"
ORIGIN
1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggttaa cttttaaaag
4021 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
4081 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta ttccagtgtg
4141 gtggaattct gcagatatcc agcacagtgg cggccgctcg acaatcaacc tctggattac
4201 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
4261 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
4321 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
4381 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
4441 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
4501 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
4561 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
4621 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
4681 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
4741 agtcggatct ccctttgggc cgcctccccg cctggaattc tgcagatatc cggactagtg
4801 atctctcgag gttaacgaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg
4861 agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg
4921 cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc
4981 gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg
5041 caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc
5101 tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc
5161 tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag
5221 gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg
5281 cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct gcatcccgcc
5341 accatgaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc
5401 gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc
5461 gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa
5521 gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac
5581 attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg
5641 ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg
5701 gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa
5761 ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg
5821 tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat
5881 gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc
5941 ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag
6001 gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg
6061 gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg
6121 ccgcggctcc ggggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
6181 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
6241 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
6301 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc
6361 aaaggaatag agtagatgcc gaccgaacaa gagctgattt cgagaacgcc tcagccagca
6421 actcgcgcga gcctagcaag gcaaatgcga gagaacggcc ttacgcttgg tggcacagtt
6481 ctcgtccaca gttcgctaag ctcgctcggc tgggtcgcgg gagggccggt cgcagtgatt
6541 caggcccttc tggattgtgt tggtccccag ggcacgattg tcatgcccac gcactcgggt
6601 gatctgactg atcccgcaga ttggagatcg ccgcccgtgc ctgccgattg ggtgcagatc
6661 cgtcgagggc ccggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
6721 ctttttaaaa gaaaaggggg gactggaagg gctagactcg agcggccgcc actgtgctgg
6781 atatcaacca ctttgtacaa gaaagctgaa cgagaaacgt aaaatgatat aaatatcaat
6841 atattaaatt agattttgca taaaaaacag actacataat actgtaaaac acaacatatc
6901 cagtcactat ggtcgacctg cagactggct gtgtataacg gagcctgaca tttatattcc
6961 ccagaacatc aggttaatgg cgtttttgat gtcattttcg cggtggctga gatcagccac
7021 ttcttccccg ataacggaga ccggcacact ggccatatcg gtggtcatca tgcgccagct
7081 ttcatccccg atatgcacca ccgggtaaag ttcacgggag actttatctg acagcagacg
7141 tgcactggcc agggggatca ccatccgtcg cccgggcgtg tcaataatat cactctgtac
7201 atccacaaac agacgataac ggctctctct tttataggtg taaaccttaa actgcatttc
7261 accagtccct gttctcgtca gcaaaagagc cgttcatttc aataaaccgg gcgacctcag
7321 ccatcccttc ctgattttcc gctttccagc gttcggcacg cagacgacgg gcttcattct
7381 gcatggttgt gcttaccaga ccggagatat tgacatcata tatgccttga gcaactgata
7441 gctgtcgctg tcaactgtca ctgtaatacg ctgcttcata gcacacctct ttttgacata
7501 cttcgggtat acatatcagt atatattctt ataccgcaaa aatcagcgcg caaatacgca
7561 tactgttatc tggcttttag taagccggat ccagatcttt acgccccgcc ctgccactca
7621 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acagacggca
7681 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
7741 atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg
7801 aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa
7861 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg
7921 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg
7981 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacgg
8041 aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg
8101 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta
8161 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat
8221 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa
8281 aatctcgccg gatcctaact caaaatccac acattatacg agccggaagc ataaagtgta
8341 aagcctgggg tgcctaatgc ggccgccata gtgactggat atgttgtgtt ttacagtatt
8401 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg
8461 tttctcgttc agcttttttg tacaaacttg ttgatatctg cagaattcca ccacactgga
8521 ctagctcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
8581 gaccagatct gagcctggga gctctctggc tgcctaggga acccactgct taagcctcaa
8641 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
8701 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
8761 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
8821 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
8881 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
8941 catgtctggc tctagctatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
9001 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
9061 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcgtcgag acgtacccaa
9121 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
9181 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
9241 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
9301 tggcgaatgg cgcgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
9361 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
9421 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
9481 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
9541 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
9601 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
9661 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
9721 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt cc
//
